Study on the adoption of Schwann cell phenotype by bone marrow stromal cells in vitro and in vivo.
- Author:
Fu-Qiang ZHAO
1
;
Pei-Xun ZHANG
;
Xiang-Jun HE
;
Chan DU
;
Zhong-Guo FU
;
Dian-Ying ZHANG
;
Bao-Guo JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers; analysis; Bone Marrow Cells; cytology; Cell Differentiation; Cells, Cultured; Flow Cytometry; Fluorescent Antibody Technique, Indirect; Fluorescent Dyes; Glial Fibrillary Acidic Protein; analysis; Morphogenesis; Organic Chemicals; analysis; Phenotype; Rats; Receptor, Nerve Growth Factor; analysis; S100 Proteins; analysis; Schwann Cells; cytology; metabolism; Sciatic Nerve; cytology; injuries; Stromal Cells; cytology; metabolism; transplantation
- From: Biomedical and Environmental Sciences 2005;18(5):326-333
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the possibilities of bone marrow stromal cells (MSCs) to adopt Schwann cell phenotype in vitro and in vivo in SD rats.
METHODSMSCs were obtained from tibia and femur bone marrow and cultured in culture flasks. Beta-mercaptoethanol followed by retinoic acid, forskolin, basic-FGF, PDGF and heregulin were added to induce differentiation of MSCs'. Schwann cell markers, p75, S-100 and GFAP were used to discriminate induced properties of MSCs' by immunofluorescent staining. PKH-67-labelled MSCs were transplanted into the mechanically injured rat sciatic nerve, and laser confocal microscopy was performed to localize the PKH67 labelled MSCs in the injured sciatic nerve two weeks after the operation. Fluorescence PKH67 attenuation rule was evaluated by flow cytometry in vitro.
RESULTSMSCs changed morphologically into cells resembling primary cultured Schwann cells after their induction in vitro. In vivo, a large number of MSCs were cumulated within the layer of epineurium around the injured nerve and expressed Schwann cell markers, p75, S-100, and GFAP.
CONCLUSIONMSCs are able to support nerve fiber regeneration and re-myelination by taking on Schwann cell function, and can be potentially used as possible substitutable cells for artificial nerve conduits to promote nerve regeneration.
