Comparative proteomic analysis of B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
- Author:
Su-Qing ZHAO
1
;
Yan-Fei CAI
;
Zhen-Yu ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; analysis; metabolism; Bartonella henselae; classification; genetics; metabolism; Electrophoresis, Gel, Two-Dimensional; Genotype; Proteomics
- From: Biomedical and Environmental Sciences 2005;18(5):341-344
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
METHODProtein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis.
RESULTSProtein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 microg/microL and 2.200 microg/microL respectively. Sample protein of 40 microg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae.
CONCLUSIONThe procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
