Fenvalerate-induced alterations in calcium homeostasis in rat ovary.
- Author:
Jun HE
1
;
Jian-Feng CHEN
;
Ru LIU
;
Lin SONG
;
Hebron C CHANG
;
Xin-Ru WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Calcium-Transporting ATPases; metabolism; Calmodulin; metabolism; Endocrine Disruptors; toxicity; Female; Homeostasis; drug effects; Insecticides; toxicity; Nitriles; toxicity; Ovary; drug effects; metabolism; pathology; Progesterone; blood; Pyrethrins; toxicity; Rats; Rats, Sprague-Dawley
- From: Biomedical and Environmental Sciences 2006;19(1):15-20
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe the effects of fenvalerate on calcium homeostasis in rat ovary.
METHODSFemale Sprague-Dawley rats were orally given fenvalerate at daily doses of 0.00, 1.91, 9.55, and 31.80 mg/kg for four weeks. The ovary ultrastucture was observed by electron microscopy. Serum free calcium concentration was measured by atomic absorption spectrophotometry. The activities of phosphorylase a in rat ovary were evaluated by the chromatometry. The total content of calmodulin in ovary was estimated by ELISA at each stage of estrous cycle. Radioimmunoassay (RIA) was used to evaluate the level of serum progesterone.
RESULTSHistopathologically, damages of ovarian corpus luteum cells were observed. An increase in serum free calcium concentration was observed in rats treated with 31.80 mg/kg fenvalerate. The activities of phosphorylase a enhanced in all treated groups, and fenvalerate increased the total content of calmodulin significantly in estrus period. Serum progesterone levels declined in fenvalerate exposed rats in diestrus.
CONCLUSIONFenvalerate interferes with calcium homeostasis in rat ovary. Also, the inhibitory effects of fenvalerate on serum progesterone levels may be mediated partly through calcium signals.
