Abnormal expression of eukaryotic translation factors in malignant transformed human bronchial epithelial cells induced by crystalline nickel sulfide.
- Author:
Yi-Xiong LEI
1
;
Xue-Min CHEN
;
Gen-Rong WU
;
Jia-Kun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers; Bronchi; cytology; Cell Line, Transformed; Cell Line, Tumor; Cell Transformation, Neoplastic; metabolism; DNA, Complementary; metabolism; Epithelial Cells; Gene Expression Regulation, Neoplastic; drug effects; Humans; Nickel; toxicity; Peptide Elongation Factor 1; genetics; metabolism; Prokaryotic Initiation Factor-3; genetics; metabolism
- From: Biomedical and Environmental Sciences 2006;19(1):53-60
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the oncogenic potential of mouse translation initiation factor 3 (TIF3) and elongation factor-1delta (TEF-1delta) in malignant transformed human bronchial epithelial cells induced by crystalline nickel sulfide (NiS).
METHODSAbnormal expressions of human TIF3 and TEF-1delta genes in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were investigated and analyzed by the reverse transcript polymerase chain reaction (RT-PCR) and fluorescent quantitative polymerase chain reaction (FQ-PCR), respectively.
RESULTSRT-PCR analysis primarily showed that both human TIF3 and TEF-1delta mRNA expressions in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were increased as compared with controls. FQ-PCR assay showed that the levels of TIF3 expressions in the transformed cells and tumorigenic cells were 3 and 4 times higher respectively, and the elevated expressions of TEF-1delta cDNA copies were 2.7- to 3.5-fold in transformed cells and 4.1- to 5.2-fold in tumorigenic cells when compared with non-transformed cells, indicating that the over-expressions of human TIF3 and TEF-1delta genes were related to malignant degree of the cells induced by nickel.
CONCLUSIONSThese findings demonstrate that there are markedly abnormal expressions of TIF3 and TEF-1delta genes during malignant transformation of human bronchial epithelial cell lines induced by crystalline NiS. They seem to be the molecular mechanisms potentially responsible for human carcinogensis due to nickel.
