Metallothionein 1 isoform gene expression induced by cadmium in human peripheral blood lymphocytes.
- Author:
Xiu-Li CHANG
1
;
Tai-Yi JIN
;
Yuan-Fen ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biomarkers; metabolism; Cadmium; pharmacology; Cells, Cultured; DNA Primers; Female; Gene Expression Regulation; drug effects; Humans; Lymphocytes; metabolism; Male; Metallothionein; genetics; metabolism; Protein Isoforms; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Biomedical and Environmental Sciences 2006;19(2):104-109
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes (HPBLs).
METHODSThe expression of mRNA representing the seven active MT-1 genes was determined in HPBLs by quantitative RT-PCR before and after exposure to cadmium.
RESULTSBasal expressions of MT-1X, and MT-1A in HPBLs were similar to expression of housekeeping gene. In contrast, the basal gene expressions of MT-1H, 1F, 1E, and 1G were a little transcripts in human HPBLs. No signal was detected for MT-1B. There was a sex difference (P < 0.05). in basal gene expression of MT-1E. The levels of gene expression of MT-1A, 1E, 1F, 1G, 1H, and 1X increased, but the level of MT-1B did not increase after exposure to cadmium.
CONCLUSIONSGene expressions of MT-1G, MT-1H, MT-1F, and MT-1X in HPBLs can be used as a potential biomarker of cadmium exposure.
