Protective effect of isoflurane and sevoflurane on ischemic neurons and expression of Bcl-2 and ICE genes in rat brain.
- Author:
Shao-Dong ZHANG
1
;
Jing ZHAI
;
Hui ZHANG
;
Hong WAN
;
De-Zhi LI
Author Information
- Publication Type:Journal Article
- MeSH: Anesthetics, Inhalation; administration & dosage; pharmacology; Animals; Apoptosis; drug effects; Brain Ischemia; drug therapy; Caspase 1; genetics; metabolism; Flow Cytometry; Hippocampus; drug effects; pathology; Isoflurane; administration & dosage; pharmacology; Male; Methyl Ethers; administration & dosage; pharmacology; Neurons; drug effects; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Rats; Rats, Wistar; Reperfusion
- From: Biomedical and Environmental Sciences 2006;19(2):143-146
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the protective effect of volatile anesthetics, isoflurane and sevoflurane, on ischemic neurons after cerebral ischemia-reperfusion in rats and its possible molecular mechanism.
METHODSRat cerebral ischemia-reperfusion model was developed by occlusion of the middle cerebral artery (MCA) and bilateral common carotid arteries (CCAs) 1 h after reperfusion. Using flow cytometry (FCM) and Northern blot hybridization, we calculated the number of apoptotic bodies and detected the expression of bcl-2 mRNA and interleukin-1beta converting enzyme (ICE) mRNA.
RESULTSThe apoptotic bodies in hippocampus analyzed by FCM peaked at appeared 24 h after reperfusion, and decreased about 54% and 40%, respectively, after treatment with isoflurane and sevoflurane, as compared with ischemic group. There was no significant difference in the expression of bcl-2 mRNA and ICE mRNA between the inhaled anesthetic groups and ischemic group in hippocampus 24 h after MCA/CCAs occlusion.
CONCLUSIONIsoflurane and sevoflurane partially inhibit apoptosis but have no significant effect on the expression of bcl-2 and ICE genes.
