Inhibitory effects of saponins from Anemarrhena asphodeloides Bunge on the growth of vascular smooth muscle cells.
- Author:
Shang-Zhi XIAO
1
;
Ming-En XU
;
Ya-Kun GE
;
Gui-Feng XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Anemarrhena; chemistry; Animals; Annexin A5; metabolism; Antineoplastic Agents, Phytogenic; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Flow Cytometry; Fluorescein; chemistry; Microscopy, Confocal; Muscle, Smooth, Vascular; cytology; drug effects; growth & development; Nitric Oxide; metabolism; Rats; Rats, Sprague-Dawley; Saponins; pharmacology
- From: Biomedical and Environmental Sciences 2006;19(3):185-191
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of saponins from Anemarrhena asphodeloides Bunge (SAaB) (Botanical Name: Anemarrhena Asphodeloidis Rhizoma) on the growth of vascular smooth muscle cells (VSMCs).
METHODSCell proliferation was measured by a newly developed cell proliferation reagent, WST-1. Cell apoptosis was assayed by flow cytometry through detecting annexin V. Nitric oxide production was evaluated using confocal laser scanning microscopy with diaminofluorescein diacetate (DAF-2, DA). Cell aldose reductase (AR) activity, as well as the effect of Epalrestat and interleukin-1beta were also explored.
RESULTSWST assay showed that cell proliferation induced by serum was significantly inhibited by SAaB (P<0.01). Flow cytometry analysis revealed that SAaB could enhance apoptotic rate of VSMCs (P<0.01). Nitric oxide production was significantly enhanced after administration of SAaB and interleukin-1beta. Moreover, AR activity of VSMCs was also remarkably inhibited by both SAaB and Epalrestat (P<0.01).
CONCLUSIONSAaB can inhibit proliferation and enhance apoptosis of VSMCs. It may protect vascular cells by inhibiting VSMC proliferation and augmenting apoptotic rate of VSMCs via NO-dependent pathway.
