Activity of Ginkgo biloba extract and quercetin on thrombomodulin expression and tissue-type plasminogen activator secretion by human umbilical vein endothelial cells.
- Author:
Wen-Jun LAN
1
;
Xiao-Xiang ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Blood Circulation; drug effects; Endothelial Cells; drug effects; Endothelium, Vascular; cytology; drug effects; Flow Cytometry; Ginkgo biloba; chemistry; Humans; Plant Extracts; pharmacology; Quercetin; pharmacology; Thrombomodulin; metabolism; Tissue Plasminogen Activator; metabolism; Umbilical Veins; cytology
- From: Biomedical and Environmental Sciences 2006;19(4):249-253
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEIn order to investigate the pharmacological properties of Ginkgo biloba extract (GBE) on improving blood circulation, the regulating action of GBE and quercetin (a main flavonoid ingredient in GBE) on thrombomodulin (TM) expression and tissue-type plasminogen activator (t-PA) secretion was studied.
METHODSUsing flow cytometer and gel image system respectively, we evaluated the TM expression and the t-PA secretion by human umbilical vein endothelial cells (HUVECs) in vitro.
RESULTSThe increase of TM expression on HUVECs surface was induced by GBE rather than quercetin in a dose- and time-dependent manner. Both GBE and quercetin increased the t-PA release significantly.
CONCLUSIONThe effect of GBE on improving blood circulation may be partly attributed to its promoting TM expression and t-PA secretion by endothelial cells, and quercetin participated in the effect of GBE on t-PA secretion. However, the action of GBE on increasing TM expression needs further study.
