High-performance liquid chromatographic determination of urinary trans, trans-muconic acid excreted by workers occupationally exposed to benzene.
- Author:
Xia-Min HU
1
;
Shi-Zhen SONG
;
Fang-Li YE
;
Li-Wen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Air Pollutants, Occupational; urine; Benzene; analysis; metabolism; Biomarkers; urine; Calibration; Chromatography, High Pressure Liquid; methods; Environmental Monitoring; methods; Humans; Metallurgy; Occupational Exposure; Reproducibility of Results; Sorbic Acid; analogs & derivatives; analysis
- From: Biomedical and Environmental Sciences 2006;19(4):292-296
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the relationship between trans, trans-muconic acid (ttMA) as benzene metabolite of occupational workers and benzene concentration in air.
METHODSA rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS (2) (5u) column (phi 4.6 mm x 150 mm) and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water (v/v, 1:2:10:87). The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals.
RESULTSA correlation coefficient (r = 0.9963) was found for ttMA ranging 0.10-10.00 microg/mL. The limit of detection was 0.10 microg/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y = 0.859 + 0.108C (before work, r = 0.6200) or Y = 1.980 + 0.179C (after work, r = 0.7930).
CONCLUSIONThis method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.
