The Effect of IGF-1 on ALP Activity of MC3T3-E1 Cell.
10.5051/jkape.1997.27.4.669
- Author:
Hu Jung LEE
1
;
Jae Mok LEE
;
Byung Ju CHOI
;
Hyun Mo YU
;
Jo Young SUH
Author Information
1. Department of Periodontology, School of Dentistry, Kyungpook National University Taegu, Korea.
- Publication Type:Original Article
- MeSH:
Alkaline Phosphatase;
Ascorbic Acid;
Cell Differentiation;
Eagles;
Insulin-Like Growth Factor I*;
Intercellular Signaling Peptides and Proteins;
Metabolism;
Osteogenesis;
Peptides
- From:The Journal of the Korean Academy of Periodontology
1997;27(4):669-684
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Polypeptide growth factors belong to a class of potent biologic mediators which regulate cell differentiation, proliferation, migration and metabolism. IGF-I is polypeptides secreted by skeletal cells and is considered as regulators of bone formation. The purpose of this study is to evaluate the effects of IGF-I on bone nodule formation and alkaline phosphatase activity of MC3T3-E1 cells. MC3T3-E1 cells were seeded at 1x10(4) cells/well, 1x10(5) cells/well in alpha-modified Eagle medium containing 10% fetal bovine serum, 10 mM beta-glycerophosphate and 50microgram/ml of ascorbic acid. Before 48 hours of indicated time, medium were changed with serum free medium. After 24 hours, 0.1, 1, 10 ng/ml IGF-I were added to the cells and cultured for 3, 7, 14, 21, 28 days. And histochemical analysis was done and ALP activity was measured and was expressed as nmol/min/mg of protein. The bone nodule formation in MC3T3-E1 cells of IGF-I was seen at 21, 28 days, but there were no difference between control group and experimental groups. The ALP activity decreased when it is compare to control 2 group except for 1 ng/ml, 10 ng/ml IGF-I of 21-day-groups and 1 ng/ml IGF-I of 28-day-groups. Dose response effects of IGF-I of ALP activity in MC3T3-E1 cells were seen the highest ALP activity at 1ng/ml until 21days and the highest ALP activity at 10 ng/ml of 28 daygroups. The peak times were seen at 7-day group, 14-day group on control group and experimental group respectively, and 1 ng/ml group was the highest ALP activity. From the above results, IGF-I was not seen notable effect on bone nodule formation and decreased ALP activity of MC3T3-E1 cells but the use of IGF-I to mediate biological stimulation of MC3T3-E1 cells shows promise for future therapeutic application.
