Effects of Substance P on the Cell Proliferation and IL-2 Production of T Lymphocyte.
10.5051/jkape.1997.27.4.805
- Author:
Jin Kyun MOON
1
;
Byung Son CHOI
;
Seok Cho LEE
;
Hyung Seop KIM
Author Information
1. Department of Periodontology, College of Dentistry, Chon-buk National University, Korea.
- Publication Type:Original Article
- MeSH:
Cell Line;
Cell Proliferation*;
Interleukin-2*;
Lymphocytes*;
Mitogens;
Nerve Endings;
Neuropeptides;
Substance P*;
T-Lymphocytes;
Tachykinins;
Up-Regulation
- From:The Journal of the Korean Academy of Periodontology
1997;27(4):805-818
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Immune responses of periodontal tissue may be regulated by products of sensory afferent nerve endings such as neuropeptides. Substance P(SP), a tachykinin neuropeptide, has been previously reported to stimulate the activities of T lymphocyte. Therefore, I examined the role of SP in IL-2 production and cell proliferation by using a homogeneous line of T lymphocytes(Jurkat and HuT78). Cell proliferation rate was determined by [3H]-thymidine incorporation test, and IL-2 was quantitated by the growth rate of CD4+ IL-2-dependent T lymphocyte line CTLL-2. SP stimulated cell proliferation of T lymphocytes at the concentration of 10(-12) and 10(-8)M in a biphasic bell-shape dose-dependent manner. However, SP alone did not induce IL-2 release at the concentration range of 10(-6) to 10(-14)M. The upregulation of IL-2 release was observed when 10(-12)M SP was applied together with mitogens such as Con A or PHA+PMA on T cell lines, especially on Jurkat. Con A or PHA+PMA demonstrated to increase the rate of cell proliferation of Jurkat, which had shown to produce much amount of IL-2 indicating that mitogen-induced cell proliferation might be partially influenced by released IL-2. It was concluded that regulatory effects of SP on the immune/inflammatory response could be mediated through the costimulatory upregulation of IL-2 production and increase of cell proliferation of T lymphocyte.
