The Expression of Human Beta Defensins by Thermal Injury.
- Author:
Hyo Sun JEONG
1
;
Cheon Jae YEON
;
Jung Suk LEE
Author Information
1. Bestian Research Center, Daejeon, Korea. nostalgie@daum.net
- Publication Type:In Vitro ; Original Article
- Keywords:
Thermal injury;
Human beta defensins;
Involucrin
- MeSH:
beta Catenin;
beta-Defensins;
Burns;
Defensins;
Humans;
Immune System;
Immunohistochemistry;
Keratinocytes;
Keratins;
Peptides;
Protein Precursors;
RNA, Messenger;
Skin;
Skin Diseases;
Wound Infection
- From:Journal of Korean Burn Society
2012;15(1):39-44
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Defensins are important components of innate immune system. These peptides have antimicrobial activity against a wise variety of pathogens that associated with burn wound infection. In particular, human beta-defensins are expressed in normal epidermal region and showed differential expression of some skin disease. We investigated that expression of human beta-defensin by in vitro and ex-vivo by thermal condition. METHODS: To investigate the expression of human beta-defensins in acute burn condition, we cultured keratinocytes and used to rat's skin at this experiment. After thermal condition, we showed the expression of beta-defensins-2 (hBD-2), -3 (hBD-3), keratins, keratinocyte differentiation and junction protein levels by RT-PCR and immunohistochemistry (IHC). RESULTS: HBD-2 & involucrin were down-regulated from 1 hr to 8 hrs in mRNA level. But others were not changed in mRNA level. In protein level, hBD-3 was decreased but pan-cytokeratin and beta-catenin were not changed. CONCLUSION: HBD-2 was down-regulated in thermal injury. Because thermal injury could induce the influence of keratinocyte differentiation and the decrease of skin protection ability. Our results suggested that human beta-defensins plays an important role in protection by several injury.
