Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells.
10.3347/kjp.2014.52.6.595
- Author:
Juan Hua QUAN
1
;
In Wook CHOI
;
Jung Bo YANG
;
Wei ZHOU
;
Guang Ho CHA
;
Yu ZHOU
;
Jae Sook RYU
;
Young Ha LEE
Author Information
1. Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Trichomonas vaginalis;
1,10-phenanthroline;
mTOR cleavage;
SiHa cell;
metalloproteinase
- MeSH:
Blotting, Western;
Cell Line, Tumor;
Epithelial Cells/metabolism/parasitology;
Humans;
Metalloproteases/genetics/*metabolism;
Proteolysis;
Sequence Analysis, DNA;
TOR Serine-Threonine Kinases/*metabolism;
Trichomonas vaginalis/*enzymology/genetics
- From:The Korean Journal of Parasitology
2014;52(6):595-603
- CountryRepublic of Korea
- Language:English
-
Abstract:
Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.
