Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment.
10.3347/kjp.2014.52.6.605
- Author:
Kyoung Ho PYO
1
;
Bong Kwang JUNG
;
Chun Feng XIN
;
You Won LEE
;
Jong Yil CHAI
;
Eun Hee SHIN
Author Information
1. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. ehshin@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
athymic mouse;
anti-tumorigenesis;
IL-12;
TIMP-1;
MyD88;
NF-kappaB
- MeSH:
Animals;
Antigens, Protozoan/*immunology;
Disease Models, Animal;
Immunity, Innate;
Immunotherapy/*methods;
Interleukin-12/*blood;
Macrophages/immunology;
Mice, Inbred BALB C;
Mice, Nude;
Myeloid Differentiation Factor 88/analysis;
Neoplasms/pathology/*therapy;
Toxoplasma/*immunology;
Treatment Outcome
- From:The Korean Journal of Parasitology
2014;52(6):605-612
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.
