The Effect of Mitomycin C(MMC) on Inhibition of Cellular Proliferation and Type_I Collagen, Laminin Synthesis of Pterygial Mesenchymal Cell.
- Author:
Boo Sup OUM
1
;
Jong Soo LEE
Author Information
1. Department of Ophthalmology, The Pusan National University Medical College.
- Publication Type:In Vitro ; Original Article
- Keywords:
a-smooth muscle actin;
Mitomycin C;
Laminin;
Mesenchymal cell;
Type-I collagen
- MeSH:
Actin Cytoskeleton;
Actins;
Basement Membrane;
Cell Proliferation*;
Collagen*;
Extracellular Matrix;
Humans;
Immunoassay;
Laminin*;
Mitomycin*;
Myofibroblasts;
Pinocytosis;
Pterygium;
Thymidine
- From:Journal of the Korean Ophthalmological Society
1999;40(3):712-720
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study is to investigate that the biological effect of mitomycin C(MMC) on inhibition of cellular proliferation, extracellular synthesis of type_I coolagen, lamini, and study of myofibroblast is derived directly from the primary and recurrent pterygial mesenchymal cell by MMC concentration and duration of exposure time used clinically. Human pterygial mesenchymal cells were exposed for 3 minutes, 5 minutes, and 10 minutes to MMC 0.01%, 0.03%, 0.05%, and DMEM(control). After cells were incubated for 24 hours, [H3] thymidine proliferative assay, immunoassay of type_I collagen and laminin, immunohistochemical and ultrastructual study of -smooth muscle actin were perfromed in vitro. Recurrent pterygal mesenchymal cells were more proliferated and stronger than primary pterygial cells in proliferation and inhibition of cellular proliferation assay. In immunoassay of extracellular matrix, the higher the concentration of MMC and longer the duration of exposure time, the inhibition of laminin are strong. However, there was a little effect of inhibition of synthesis of type-I collagen. Also the results of positive responsed immunohistochemical and ultrastructual finding such as a few pinocytosis, microfilaments, microtendon, and basal lamina like materal by TEM of myofibroblast were revealed. We think that the reccurent pterygial tissue have more effect on inhibition of cellular proliferation and laminin synthesis than primary pterygium. Therefore, reconsideration of MMC concentreation and duration time should be need in case of recurrent tissue, further experimental and clinical research on the myofibroblast and inhibition of type-I collagen also should be need.
