Presence of E-cadherin in Cultured Amnionic Cells.
- Author:
Ki Hwan LEE
;
Young Suk SEO
;
Kil Chun KANG
- Publication Type:Original Article
- Keywords:
E-cadherin;
Amnion;
Culture
- MeSH:
Amnion*;
Cadherins*;
Cell Adhesion Molecules;
Cesarean Section;
Cytoplasm;
Female;
Humans;
Immunohistochemistry;
Membranes;
Pregnancy;
RNA, Messenger
- From:Korean Journal of Obstetrics and Gynecology
1999;42(12):2690-2694
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Cell adhesion molecules may play a role in integrating amnionic membrane. The objective of this study was to prove E-cadherin mRNA and proteins in cultured human amnionic cells. METHODS: We cultured amnionic cells from 4 women undergoing cesarean section without labor. E-cadherin was determined by immunohistochemistry and Western ligend blotting. To demonstrate E-cadherin mRNA, reverse transcription-polymerase chain reaction was performed. RESULTS: On immunohistochemistry, E-cadherin was abundantly showed on the cytoplasm of the cells. Western ligend blotting showed clear 120 kDa bands on four specimens, and relatively weak band on one specimen. Reverse transcription-polymerase chain reaction showed 432 BP bands. CONCLUSION: We proved E-cadherin and its mRNA by immunohistochemistry, Western ligend blotting and reverse transcription-polymerase chain reaction in cultured human amnionic cells.
