Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells.
10.5395/rde.2015.40.4.290
- Author:
Minjeong PARK
1
;
Nan Sim PANG
;
Il Young JUNG
Author Information
1. Department of Conservative Dentistry, Yonsei University College of Dentistry, Seoul, Korea. juen@yuhs.ac
- Publication Type:Original Article
- Keywords:
Calcium hydroxide;
Cell attachment;
Cell differentiation;
Dental pulp stem cells;
Regenerative endodontics;
Sodium hypochlorite
- MeSH:
Calcium Hydroxide;
Cell Adhesion;
Cell Differentiation;
Cell Survival;
Dental Pulp*;
Dentin*;
Edetic Acid;
Gene Expression;
Humans*;
Microscopy, Electron, Scanning;
Molar, Third;
Real-Time Polymerase Chain Reaction;
Sodium Hypochlorite;
Stem Cells*
- From:Restorative Dentistry & Endodontics
2015;40(4):290-298
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and Ca[OH]2 application on the attachment and differentiation of dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL Ca[OH]2, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. RESULTS: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the Ca[OH]2- and the EDTA-treated groups were significantly higher than those in the other groups. All Ca[OH]2-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both Ca[OH]2 and EDTA. CONCLUSIONS: The application of Ca[OH]2 and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment.
