Gene Expression Pattern of Human Chorion-Derived Mesenchymal Stem Cells during Adipogenic Differentiation.
10.3349/ymj.2012.53.5.1036
- Author:
Yeon Hee KIM
1
;
Tae Chul PARK
;
Guisera LEE
;
Jong Chul SHIN
Author Information
1. Department of Obstetrics and Gynecology, Uijeongbu St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Uijeongbu, Korea.
- Publication Type:Original Article ; In Vitro
- Keywords:
Chorion;
mesenchymal stem cells;
adipogenesis;
differentiation;
gene expression
- MeSH:
Activated-Leukocyte Cell Adhesion Molecule;
Adipocytes;
Adipogenesis;
Carrier Proteins;
Chorion;
Female;
Fibroblasts;
Flow Cytometry;
Gene Expression*;
Glycerolphosphate Dehydrogenase;
HLA-DR Antigens;
Humans*;
Mesenchymal Stromal Cells*;
Parturition;
Placenta;
PPAR gamma;
Pregnancy;
Pregnancy Trimester, Third;
RNA
- From:Yonsei Medical Journal
2012;53(5):1036-1044
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to identify the adipocyte-specific gene expression patterns in chorion-derived mesenchymal stem cells during adipogenic differentiation. MATERIALS AND METHODS: Chorionic cells were isolated from the third trimester chorions from human placenta at birth and identified morphologically and by fluorescence-activated cell sorting analysis. After inducing adipogenic differentiation for 28 days, cells at days 3, 10, 21 and 28 were analyzed by Oil red O staining and RNA extraction in order to assess the expression levels of adipocyte marker genes, including CCAAT-enhancer binding protein alpha (C/EBPalpha), peroxisome proliferator-activated receptor gamma (PPARgamma), fatty acid binding protein 4 (FABP4) and Glycerol-3-phosphate dehydrogenase (GPD2). Cells not induced for differentiation were compared with the induced cells as a control group. RESULTS: Chorion-derived cells showed the same pattern as fibroblasts, and expressed CD73, CD105, and CD166 antigens, but not CD45, CD34, and HLA-DR antigens. On day 3 after differentiation, cells began to stain positively upon Oil red O staining, and continuously increased in lipid granules for 4 weeks. The expression level of C/EBPalpha increased 4.6 fold on day 3 after induction, and continued to increase for 4 weeks. PPARgamma was expressed at a maximum of 2.9 fold on day 21. FABP4 and GPD2 were significantly expressed at 4.7- and 3.0-fold, respectively, on day 21, compared to controls, and further increased thereafter. CONCLUSION: Human chorion-derived mesenchymal stem cells exhibited the sequential expression pattern of adipocyte marker genes during differentiation, corresponding to adipogenesis.
