Use of Consensus Primer Set, GP5+ and GP6+, to Detect Human Papillomavirus within Genital Lesions.
- Author:
Gil Ho LEE
1
Author Information
1. Department of Urology, Dankook University College of Medicine, Cheonan, Korea. gilho@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Papillomavirus;
Human;
Consensus;
Primer;
Genitalia
- MeSH:
Carcinoma, Squamous Cell;
Carcinoma, Verrucous;
Condylomata Acuminata;
Consensus*;
DNA;
Genitalia;
Genotype;
Humans*;
Polymerase Chain Reaction;
Population Characteristics;
Prevalence
- From:Korean Journal of Urology
2003;44(6):606-610
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The human papillomavirus (HPV) group comprises over 90 different genotypes, of which more than 30 are mucosotropic. HPV types 16 and 18 are considered the major cause of invasive carcinomas and their related precursors. However, other types are also responsible for the development of carcinomas, condylomas acuminatas and genital warts. The prevalence of the different HPV genotypes in the general population shows heterogeneity and geographical variations. Therefore, a consensus primer set was evaluated to detect HPV in patients with genital lesions. MATERIALS AND METHODS: Genomic DNA was extracted from 2 penile squamous cell carcinomas, 2 verrucous carcinomas, 11 condyloma acuminatas and 2 suspicious lesions. A general primer GP5+ /GP6+ mediated PCR was performed. RESULTS: All the condyloma acuminata, carcinoma and suspicious lesions showed a 150 bp PCR products with the consensus primer GP5+ /GP6+ . CONCLUSIONS: The PCR method, with a general primer set, GP5+ /GP6+ , can detect mucosotropic HPV from various genital lesions. It may be a broad spectrum PCR method, but it can also detect small amounts HPV from suspicious lesions.
