Effect of ovarian on FSH release and subunit synthesis in rat anterior pituitary cells in culture.
- Author:
You Kon KIM
;
Kyung Hwan KIM
- Publication Type:In Vitro ; Original Article
- Keywords:
GnRH;
Estrogen;
Progesterone;
FSH;
RU486
- MeSH:
Animals;
Estradiol;
Estrogens;
Gonadotropin-Releasing Hormone;
Mifepristone;
Progesterone;
Rats*;
RNA, Messenger;
Steroids
- From:Korean Journal of Obstetrics and Gynecology
1999;42(1):41-47
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: to determine whether ovarian steroids directly regulate FSH release and B subunit synthesis at the pituitary level METHODS: In vitro study. After applying ovarian steroids in cultured rat anterior pituitary cells, we evaluate the amount of relesed FSH and FSH B subunit using dot-blot hybridization. RESULTS: Estradiol alone or GnRH-induced did not modulate FSH release and B ubunit synthesis. Although progesterone alone did not alter FSH release, GnRH-induced FSH release was significantly augmented by the treatment of progesterone. Progesterone alone stimulated FSH B mRNA level. Significant increase in FSH B mRNA level was also observed by the combined treatment of progesterone and GnRH. The effects of progesterone on GnRH-induced FSH release was significantly reduced by the treatment of progesterone antagonist, RU 486. The treatment with progesterone antagonist, RU 486 also abolished progesterone-induced FSH B subunit mRNA biosynthesis. CONCLUSION: From these results, it is, therefore, concluded that estradiol does not seem to be a major regulator for FSH synthesis but progesterone may exert its action at the pituitary level for the synthesis and release of FSH.
