Comparison of estrogen receptor-alpha, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids.
10.5468/ogs.2014.57.2.144
- Author:
Eun Hee KIM
1
;
Joo Young KIM
;
Yoon Hee LEE
;
Gun Oh CHONG
;
Ji Young PARK
;
Dae Gy HONG
Author Information
1. Gynecologic Cancer Center, Kyungpook National University Medical Center, Daegu, Korea. chssa0220@hanmail.net
- Publication Type:Original Article
- Keywords:
Calponin;
Estrogen receptor;
Gonadotropin-releasing hormone agonist;
Leiomyoma;
Progesterone receptor
- MeSH:
Antibodies;
Estrogens*;
Female;
Humans;
Leiomyoma*;
Progesterone*;
Receptors, Progesterone*;
Retrospective Studies
- From:Obstetrics & Gynecology Science
2014;57(2):144-150
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was aimed to compare immunohistochemical expression of estrogen receptor (ER)-alpha, progesterone receptor (PR), and calponin in gonadotrophin-releasing hormone agonist (GnRH-a)-sensitive and -resistant uterine fibroids. METHODS: We collected data retrospectively. The sensitive group consisted of women who had reduction in uterine volume greater than 40% following GnRH-a treatment. Uterine volume was either reduced by less than 10%, or was increased in the resistant group. A tissue microarray was constructed using formalin-fixed, paraffin-embedded tissues, 31 and 26 patients for the sensitive and resistant groups, respectively. Tissue sections were immunostained with antibodies against ER-alpha, PR, and calponin. The intensity and area of the immunohistochemical reactions were evaluated using a semi-quantitative scoring system. The Mann-Whitney U-test, Fisher's exact test, and Spearman's rank correlation test were used for analysis of data. RESULTS: PR (P = 0.04) and calponin (P = 0.03) showed a significantly higher staining intensity in the resistant group than in the sensitive group. Both groups showed comparable expression of ER-alpha (P = 0.23). In correlation analysis between changes in uterine volume after GnRH-a therapy and clinicopathological factors, the immunohistochemical intensity of PR (P = 0.04) and calponin (P = 0.03) was significantly correlated with changes in uterine volume. CONCLUSION: This study shows that GnRH-a resistance of uterine fibroids is not related to ER-alpha content, but the expression of PR and calponin is related with GnRH-a resistance.
