Efflux Pump Inhibitor Carbonyl Cyanide-m-chlorophenylhydrazone (CCCP) Enhances Bacteriostatic Activity of Trimethoprim-sulfamethoxazole Against Clinical Stenotrophomonas maltophilia Isolates from Korea.
10.4167/jbv.2016.46.4.185
- Author:
Ji Young RHEE
1
;
Ji Young CHOI
;
Kwan Soo KO
Author Information
1. Division of Infectious Diseases, Department of Medicine, Dankook University, Cheonan, Korea.
- Publication Type:Original Article
- Keywords:
Stenotrophomonas maltophilia;
Co-trimoxazole;
Efflux pump
- MeSH:
2,4-Dinitrophenol;
Anti-Bacterial Agents;
Carbonyl Cyanide m-Chlorophenyl Hydrazone;
Korea*;
Microbial Sensitivity Tests;
Protons;
Reserpine;
Stenotrophomonas maltophilia*;
Stenotrophomonas*;
Thiram*;
Trimethoprim, Sulfamethoxazole Drug Combination*
- From:Journal of Bacteriology and Virology
2016;46(4):185-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
Although trimethoprim-sulfamethoxazole (TMP-SXT) is considered the first-line therapy for Stenotrophomonas maltophilia infections, there is debate on the use of the bacteriostatic drug in serious infections, and recently, there has been an increasing occurrence of acquired resistance to TMP-SXT. In the present study, the effect of efflux pump inhibitors on the susceptibility of TMP-SXT and other antibiotics were investigated in S. maltophilia complex. The sul and/or dfrA genes were identified in only up to 27.8% of all 36 TMP-SXT-resistant S. maltophilia complex isolates. Thus, TMP-SXT resistance in S. maltophilia was not explained completely by the presence of sul and dfrA genes. Carbonyl cyanide-m-chlorophenylhydrazone (CCCP) decreased the minimum inhibitory concentration (MIC) of TMP-SXT by eight to 128 folds in all 14 isolates. In contrast, 2,4-dinitrophenol (DNP), phenyl-arginine-β-naphthylamide (PAβN), and reserpine did not reduce the MIC of TMP-SXT. In addition to TMP-SXT, slight decrease in MICs was observed for tigecycline and piperacillin/tazobactam by CCCP (by two folds) in one isolate. Although efflux pump may play a role in TMP-SXT resistance in S. maltophilia, inhibition of the efflux pump could be done by active proton pore.
