Isolation and Genetic Characterization of Orientia tsutsugamushi from Scrub Typhus Patients in Gyeongsangnam-do, Korea.
10.4167/jbv.2016.46.4.275
- Author:
Jong Hun HA
1
;
Dong Hae LEE
;
Jin Sik PARK
;
Oh Hyun CHO
;
Dong Hoon KIM
;
Na Young HA
;
Nam Hyuck CHO
;
Kyu Jam HWANG
;
Yong Gon CHO
;
Hye Soo LEE
;
Won Kil LEE
;
Yoo Chul LEE
;
Myung Je CHO
;
Woo Kon LEE
;
Min Kyoung SHIN
Author Information
1. Department of Microbiology, Gyeongsang National University School of Medicine, Jinju, Korea. wklee@gnu.kr mkshin@gnu.kr
- Publication Type:Original Article
- Keywords:
Scrub typhus;
Orientia tsutsugamushi;
56-kDa type-specific antigen;
tsa56;
West Gyeongsangnam-do
- MeSH:
Amino Acid Sequence;
Base Sequence;
Chungcheongnam-do;
Epidemiology;
Genotype;
Gyeongsangnam-do*;
Humans;
Incidence;
Korea*;
Membrane Proteins;
Orientia tsutsugamushi*;
Rodentia;
Scrub Typhus*;
Trombiculidae
- From:Journal of Bacteriology and Virology
2016;46(4):275-282
- CountryRepublic of Korea
- Language:English
-
Abstract:
Orientia tsutsugamushi (O. tsutsugamushi), which is endemic to an Asia-Pacific region, has increased its incidence and caused annually around 10 thousand patients infected with scrub typhus in Korea in the past several years. In the present study, we isolated 44 O. tsutsugamushi from the patients with febrile illness accompanied with or without an eschar in Gyeongsangnam-do, Korea. These isolates were characterized by genetic analysis of the major outer membrane protein, the 56-kDa type-specific antigen (tsa56), which is unique to O. tsutsugamushi. Two types of sequences of tsa56, designated by JJ1 and JJ2, were determined from 37 and 7 isolates of the 44 isolates, respectively. JJ1 and JJ2 showed 74.7~90.8% identity in nucleotide sequence and 66.1~90.5% identity in amino acid sequence with 33 reference strains except for Boryong and Kuroki. JJ1 and JJ2 had 100 and 99.9% nucleotide identity to Boryong strain, and 99.9 and 99.8% to Kuroki, which has been known to be similar to Boryong, respectively. In addition, they showed 77.9~ 81.4% nucleotide identity with the cluster of Gilliam-related genotypes, whereas they showed higher nucleotide identity (89.6~90.8%) with the cluster of Karp-related genotypes. To our knowledge, this is the first report to isolate O. tsutsugamushi and characterize their genotype as the Boryong in Jinju and West Gyeongsangnam-do, Korea, even though it has been reported that the Boryong was the predominant genotype in isolates from chiggers, domestic rodents, and patients in the southern part of Korea. Furthermore, our isolates could be useful source to study on the pathophysiology and epidemiology of scrub typhus in Korea.
