Effect of Nitric Oxide on the Proliferation of Cultured Human Tenon Capsule Fibroblasts Exposed to Benzalkonium Chloride.
- Author:
Jae Woo KIM
1
;
Keun Hae KIM
;
Beum Joon CHO
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Benzalkonium chloride;
Fibroblast;
L-NAME;
Nitric oxide;
Sodium nitroprusside
- MeSH:
Benzalkonium Compounds*;
Cell Proliferation;
Fibroblasts*;
Humans*;
NG-Nitroarginine Methyl Ester;
Nitric Oxide Synthase;
Nitric Oxide*;
Nitroprusside;
Tenon Capsule*;
Tissue Donors
- From:Journal of the Korean Ophthalmological Society
2003;44(8):1908-1913
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate whether the effect of benzalkonium chloride (BAK) on the proliferation of human Tenon capsule fibroblasts (HTCF) is mediated by nitric oxide (NO) in tissue culture . METHODS: HTCF were exposed to various concentrations of BAK for 24 hr and the cellular proliferation and production of NO were assessed by MTT and Griess assays, respectively. A non-specific nitric oxide synthase inhibitor, 0, 5 mM N(omega)-Nitro-L-arginine methyl ester (L-NAME), or NO donor, 100 micro M sodium nitroprusside (SNP), was co-administered with BAK to assess the effect of NO on the proliferation of cells. RESULTS: BAK decreased cellular proliferation in a dose-dependent manner and this effect was accompanied with decreased nitrite production. The cellular proliferation was increased significantly with BAK/SNP co-administration but decreased with BAK/L-NAME co-administration. CONCLUSIONS: NO donor increased the proliferation of HTCF which was decreased by BAK. The effect of BAK on the proliferation of HTCF may be possibly mediated by NO.
