Preliminary study on P53 gene in peripheral blood lymphocyte of veterans from dioxin-exposed regions in Vietnam
- Author:
Anh The Bui
- Publication Type:Journal Article
- Keywords:
P53 gene
- MeSH:
Genes, p53;
Veterans;
Lymphocytes;
Dioxins
- From:Journal of Medical Research
2002;18(2):29-34
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
In Vietnam war, US army applied a great amount of herbicides (especially the agent orange) which was contaminated with a very poisonous chemical named dioxin. There were a high prevalence of some cancers among people who were exposed to dioxin. In oncology, the p53 gene plays an important role as a tumor suppression gene. This study was carried out to investigate the p53 gene in peripheral blood lymphocyte from a number of veterans who served in dioxin exposed regions in Vietnam. The 1st group (exposed group) include 25 veterans who served in the dioxin exposed regions in Vietnam for at least three years. The 2nd group (control group) include 15 veterans who served only in Northern Vietnam. DNA samples were extracted from peripheral blood lymphocyte. PCR was carried out for 35 cycles with the specific primers P18-M10. PCR products were checked by electrophoresis. The results showed that: exposed group: The frequency of the investigated p53 gene is 40% (10/25 samples). Control group: the frequency of the investigated p53 gene is 73.3% (11/15 samples). There is a significant difference between the frequency of the p53 gene in the group of dioxin exposed veterans and that of the control group (p<0.05).
