Changes in Caspase-3, B Cell Leukemia/Lymphoma-2, Interleukin-6, Tumor Necrosis Factor-alpha and Vascular Endothelial Growth Factor Gene Expression after Human Umbilical Cord Blood Derived Mesenchymal Stem Cells Transfusion in Pulmonary Hypertension Rat M.
10.4070/kcj.2016.46.1.79
- Author:
Kwan Chang KIM
;
Jae Chul LEE
;
Hyeryon LEE
;
Min Sun CHO
;
Soo Jin CHOI
;
Young Mi HONG
- Publication Type:Original Article
- Keywords:
Hypertension, pulmonary;
Stem cell;
Vascular remodeling;
Inflammation;
Apoptosis
- MeSH:
Animals;
Apoptosis;
Caspase 3*;
Collagen;
Fetal Blood*;
Gene Expression*;
Heart;
Humans*;
Hypertension;
Hypertension, Pulmonary*;
In Situ Nick-End Labeling;
Inflammation;
Interleukin-6*;
Interleukins;
Lung;
Mesenchymal Stromal Cells*;
Models, Animal*;
Monocrotaline;
Muscle, Smooth, Vascular;
Natriuretic Peptide, Brain;
Rats*;
RNA, Messenger;
Stem Cells;
Tumor Necrosis Factor-alpha*;
Umbilical Cord*;
Vascular Endothelial Growth Factor A*
- From:Korean Circulation Journal
2016;46(1):79-92
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Failure of vascular smooth muscle apoptosis and inflammatory response in pulmonary arterial hypertension (PAH) is a current research focus. The goals of this study were to determine changes in select gene expressions in monocrotaline (MCT)-induced PAH rat models after human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) transfusion. MATERIALS AND METHODS: The rats were separated into 3 groups i.e., control group (C group), M group (MCT 60 mg/kg), and U group (hUCB-MSCs transfusion) a week after MCT injection. RESULTS: TUNEL assay showed that the U group had significantly lowered positive apoptotic cells in the lung tissues, as compared with the M group. mRNA of caspase-3, B cell leukemia/lymphoma (Bcl)-2, interleukin (IL)-6, tumor necrosis factor (TNF)-alpha and vascular endothelial growth factor (VEGF) in the lung tissues were greatly reduced at week 4 in the U group. Immunohistochemical staining of the lung tissues also demonstrated a similar pattern, with the exception of IL-6. The protein expression of caspase-3, Bcl-2 VEGF, IL-6, TNF-alpha and brain natriuretic peptide in the heart tissues were significantly lower in the U group, as compared with the M group at week 2. Furthermore, the protein expression of VEGF, IL-6 and BNP in the heart tissues were significantly lower in the U group at week 4. Collagen content in the heart tissues was significantly lower in the U group, as compared with M group at weeks 2 and 4, respectively. CONCLUSION: hUCB-MSCs could prevent inflammation, apoptosis and remodeling in MCT-induced PAH rat models.
