Expression of Extracellular Matrix, c-Ha-ras and c-myc genes in Cultured Pterygial Fibroblasts.
- Author:
Kwang Soo KIM
1
;
Sang Ha KIM
;
Seong Il SUH
;
Min Ho SUH
Author Information
1. Department of Ophthalmology, Keimmyung University School of Medicine, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Extrcelular matrix genes;
c-Ha-ras;
c-myc;
Northern;
slot;
dot blotting;
Pterygium
- MeSH:
Conjunctiva;
Cornea;
Extracellular Matrix*;
Fibroblasts*;
Fibronectins;
Genes, myc*;
Oncogenes;
Procollagen;
Pterygium;
RNA
- From:Journal of the Korean Ophthalmological Society
1991;32(11):929-938
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Pterygium, a disease of unknown origin and pathogennesis, is a chronic condition characterized by the encroachment of triangular portion of the bulbar conjunctiva onto the cornea. We have studied the expression of extracellular matrix genes and oncogenes in cultured pterygium using Northernm dot, and slot blot hybridizations. Northern hybridization with total RNA isolated from passaged (4-8 passages) cultures demonstrated expression of genes for alpha1(I) and alpha1(III) procollagen, fibronectin, and c-Ha-ras, but no expression of gene for c-myc was observed. The pterygium exhibited significantly increased expression of alpha1(I) and alphal(III) procollagen genes when compared with normal control cells(p<0.01). And We observed there were no differences between pterygium and normal control cells in the expression of genes for fibronectin and c-Ha-ras. According to these results we thought that the causes of pterygium may be related to the increased expression of alpha1(I) and alphal(III) procollagen genes but may not be related to c-Ha-ras, c-myc, and fibronectin genes.
