Expression of mRNA for Monocyte Chemoattractant Protein-1 in the Sciatic Nerve and Hindlimb Muscle Following Denervation.
- Author:
Jeong Hoon SONG
1
;
Young Cheon NA
;
Myoung Ae CHOI
;
Min Sun KIM
;
Byung Rim PARK
Author Information
1. Department of Plastic and Reconstructive Surgery, School of Medicine, Wonkwang University, Iksan, Korea. ycnadr@wmc.wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
MCP-1;
Sciatic nerve;
Hindlimb muscle;
alpha-Lipoic acid
- MeSH:
Animals;
Atrophy;
Chemokine CCL2*;
Defense Mechanisms;
Denervation*;
Diabetic Neuropathies;
Hindlimb*;
Humans;
Leukocytes;
Monocytes*;
Muscle, Skeletal;
Muscles;
Rats;
Rats, Sprague-Dawley;
RNA, Messenger*;
Sciatic Nerve*;
Thioctic Acid
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2003;30(5):628-634
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Migration of inflammatory cells into damaged tissue is essential to host defense mechanisms and immune responses. Monocyte chemoattractant protein-1(MCP-1), a member of the CC or beta-chemokine family is a powerful leukocyte recruitment factor that is relatively specific for monocyte/macrophage. The purpose of present study was to evaluate temporal change of expression of mRNA for MCP-1 in the sciatic nerve and hindlimb muscle of Sprague-Dawley rat. The mid-portion of the sciatic nerve was exposed, cut under aseptic condition and then animals were sacrificed 1, 2, 3, 7, 14 days after denervation of the sciatic nerve. Semiquantitative RT-PCR method and image analysis system were used to analyze change in expression of mRNA for MCP-1. Expression of mRNA for MCP-1 in the distal stump of the denervated sciatic nerve was up-regulated within 1 day and peaked on the second day following transection of the sciatic nerve. Thereafter high expression of mRNA for MCP-1 lasted for 7 days after transection of the nerve. However the expression of mRNA for MCP-1 was rapidly decreased on the 14th day after denervation. Temporal change of expression of mRNA for MCP-1 in the proximal portion of the denervated sciatic nerve was very similar with that of distal one. The gastrocnemius muscle also showed significant increase in expression of mRNA for MCP-1 on the 1st day with maximal expression on the 7th day after denervation of the sciatic nerve. In contrast, the expression of mRNA for MCP-1 in the soleus muscle was lower than the gastrocnemius muscle in the course of the denervation-induced atrophy. Additionally alpha-lipoic acid, a potent antioxidant that is used in treatment for diabetic neuropathy, suppressed significantly expression of mRNA for MCP-1 in the denervated sciatic nerve but not in atrophied hindlimb muscles.
