Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector.
- Author:
Kyul JO
1
;
Hee Jung CHO
;
Hee YI
;
Soo Min CHO
;
Jin A PARK
;
Chan Hyeok KWON
;
Hee Ra PARK
;
Ki Sung KWON
;
Ho Chul SHIN
Author Information
- Publication Type:Original Article
- Keywords: Determination; oxyclozanide; beef; milk; HPLC
- MeSH: Acetonitriles; Chromatography, High Pressure Liquid; Chromatography, Liquid; Dehydration; Formates; Milk; Nitrogen; Oxyclozanide; Phosphoric Acids; Rivers; Sodium; Sulfates; Syringes
- From:Laboratory Animal Research 2011;27(1):37-40
- CountryRepublic of Korea
- Language:English
- Abstract: This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C18, 4.6x250 mm, 5 microm) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (60:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 microm syringe filter. This method had good selectivity and recovery (70.70+/-7.90-110.79+/-14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 microg/kg for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
