Focal Cerebral Ischemia Reduces Protein Phosphatase 2A Subunit B Expression in Brain Tissue and HT22 Cells.
- Author:
Phil Ok KOH
1
Author Information
- Publication Type:Letter
- Keywords: Glutamate; middle cerebral artery occlusion; PP2A subunit B
- MeSH: Adult; Animals; Apoptosis; Blotting, Western; Brain; Brain Injuries; Brain Ischemia; Cell Cycle; Cell Death; Cell Line; Glutamic Acid; Humans; Infarction, Middle Cerebral Artery; Male; Middle Cerebral Artery; Neurons; Protein Phosphatase 2; Rats; Serine; Threonine
- From:Laboratory Animal Research 2011;27(1):73-76
- CountryRepublic of Korea
- Language:English
- Abstract: Protein phosphatase 2A (PP2A) is a serine and threonine protein phosphatase that regulates cell cycle progression and apoptosis. PP2A is composed of various subunits. Among these subunits, subunit B plays an important role in the modulation of PP2A function in the brain. This study investigated PP2A subunit B expression levels after neuronal cell injury. Middle cerebral artery occlusions (MCAO) were surgically induced in adult male rats to induce focal cerebral ischemic injury, and brain tissues were collected 24 h after MCAO. A proteomic approach revealed reduction of PP2A subunit B protein spots in MCAO-operated animals in comparison to sham-operated animals. Western blot analysis confirmed that MCAO induces reductions in PP2A subunit B levels. Moreover, glutamate exposure induces neuronal cell death and leads to reductions of PP2A subunit B levels in a hippocampal-derived cell line. This study demonstrated the decrease of PP2A subunit B in ischemic neuronal cell injury. These results suggest that the decrease of PP2A subunit B after ischemic brain injury can mediate neuronal cell death.
