Evaluation of HUBI-QUANPRO Point-of-Care Testing for Cardiac Markers.
- Author:
Jinyoung YANG
1
;
Hyojin CHAE
;
Jehoon LEE
;
Yonggoo KIM
;
Soo Young KIM
;
Hae Kyung LEE
;
Hi Jeong KWON
;
Yeongsic KIM
Author Information
1. Catholic Lab Development & Evaluation Center, Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. yeongsik@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
CK-MB;
Cardiac troponin I;
Brain natriuretic peptide (BNP);
Point-of-care testing (POCT)
- MeSH:
Creatine Kinase;
Emergencies;
Gold Colloid;
Hemoglobins;
Hemolysis;
Korea;
Natriuretic Peptides;
Triage;
Troponin I
- From:Journal of Laboratory Medicine and Quality Assurance
2011;33(2):75-82
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recently, quantitative point-of-care testing (POCT) for cardiac markers using colloidal gold particles was developed in Korea. We evaluated the analytical performance of the HUBI-QUANPRO (Humasis, Korea) assay in comparison with two other assays. METHODS: We evaluated the analytical precision and linearity of HUBI-QUANPRO creatine kinase (CK)-MB, cardiac troponin I (cTnI), and B-type natriuretic peptides (BNP). HUBI-QUANPRO assay was compared with ADVIA Centaur (Siemens, Germany) and Triage (Biosite Diagnostics, USA) assays by using 100 blood samples. In addition, we evaluated the interference of hemoglobin on the HUBI-QUANPRO assay. RESULTS: The coefficients of variation of HUBI-QUANPRO CK-MB, cTnI, and BNP were 7.5-9.7%, 12.0-17.4%, and 14.7-15.7%, respectively. The linearity ranges of HUBI-QUANPRO CK-MB, cTnI, and BNP were 4.7-27.8 ng/mL, 0.76-6.51 ng/mL, and 76.2-762.2 ng/mL, respectively. The comparison study showed no significant difference among them. When 0.5% hemolysis occurred, remarkable hemoglobin interference was found in the three markers resulting in underestimation of the concentrations. CONCLUSIONS: HUBI-QUANPRO CK-MB and BNP showed good analytical performances compared with the other two assays. Hemoglobin interference was noted in the HUBI-QUANPRO assay, especially more in BNP. Although the linearity range of cTnI was narrow, its agreement rate with ADVIA Centaur was good, thus the HUBI-QUANPRO assay could be useful as a quantitative POCT for cardiac markers in the emergency department.
