K-ras and p53 gene mutation in endometrial hyperplasia and adenocarcinoma.
- Author:
Hye Ok KIM
;
Joo Han LEE
;
In Woo LEE
;
Sun Aee HAN
;
Mee Ja PARK
;
Hyun Deuk CHO
;
In Sun KIM
;
Chul Hwan KIM
- Publication Type:Original Article
- Keywords:
K-ras, p53;
endometrial hyperplasia;
adenocarcinoma
- MeSH:
Adenocarcinoma*;
Carcinogenesis;
Codon;
Endometrial Hyperplasia*;
Endometrium;
Exons;
Female;
Genes, p53*;
Humans;
Hyperplasia;
Lymph Nodes;
Neoplasm Metastasis;
Point Mutation
- From:Korean Journal of Obstetrics and Gynecology
1999;42(10):2192-2198
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Endometrial adenocarcinoma is the third common malignancy of female genital tract and categorized as estrogen-dependent tumor (type I) or not (type II). Type II endometrial adenocarcinoma more frequently occurs in oriental, which may arise from genetic alterations such as K-ras and p53. To identify whether the K-ras and p53 mutational activation are occurred during endometrial carcinogenesis, we examined point mutations of K-ras codon 12, 13, 61 (PCR-RFLP) and p53 exon 5, 6, 7, 8 (PCR-SSCP) in the 19 samples of endometrial adenocarcinoma. The 9 samples of normal endometrium and 24 samples of endometrial hyperplasia were also examined. K-ras codon 12 mutations were found in one of 3 cases of atypical hyperplasia (33.3%) and three of 19 cases of endometrial adenocarcinoma (15.7%). The correlation with K-ras mutation and endometrial hyperplasia/adenocarcinoma was statistically insignificant(p=0.306). p53 mutation was found in only one case of endometrial adenocarcinoma which concomitantly occurred with K-ras mutation. It could not be determined that K-ras mutation was early or late event during endometrial carcinogenesis due to a few cases of atypical hyperplasia and no K-ras mutation in the benign endometrial hyperplasia. The endometrial adenocarcinoma with K-ras mutation was observed in postmenopausal old age groups, and revealed moderate to severe histologic grade, early clinical stage, and negative lymph node metastasis.
