Role of annexin-I in cervical cancer cell proliferation.
- Author:
Young Ok LEW
;
Tae Chul PARK
;
Sung Eun NAMKOONG
;
Dong Choon PARK
;
Jae Hoon KIM
;
Dae Hoon KIM
;
Yong Jae YANG
;
Jee Hyun LEE
;
Sung Dae CHO
;
Hyeong Kwon JO
- Publication Type:Original Article
- Keywords:
Annexin-I;
Cervical cancer;
Proliferation
- MeSH:
Antibodies;
Blotting, Western;
Cell Line;
Cell Proliferation*;
Coloring Agents;
Cytoplasm;
Estradiol;
HeLa Cells;
Humans;
Tamoxifen;
Tretinoin;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
1999;42(10):2199-2204
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. RESULTS: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and beta- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. CONCLUSIONS: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.
