A study of the effects of electric current on the mineralzation of the cultured calvaria bone cells.
10.5051/jkape.1997.27.4.949
- Author:
Joon Bong PARK
1
;
In Sik HUR
;
Hye Ja LEE
;
Young Chul CHOI
Author Information
1. Department of Periodontics, Dental College, Kyunghee University, Korea.
- Publication Type:In Vitro ; Original Article
- MeSH:
Alkaline Phosphatase;
Animals;
Biological Factors;
Cell Proliferation;
Guided Tissue Regeneration;
Intercellular Signaling Peptides and Proteins;
Osteoblasts;
Osteogenesis;
Periodontal Diseases;
Rats;
Regeneration;
Skull*;
Transplants
- From:The Journal of the Korean Academy of Periodontology
1997;27(4):949-961
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30HZ at 10micronA) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities (5x10(4) cells/cm2) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.
