Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development.
10.5653/cerm.2017.44.4.193
- Author:
Hye Jin KIM
1
;
Sung Baek PARK
;
Jung Bo YANG
;
Young Bae CHOI
;
Ki Hwan LEE
Author Information
1. Department of Obstetrics and Gynecology, Chungnam National University Hospital, Daejeon, Korea. good79ok@naver.com
- Publication Type:Original Article
- Keywords:
Blastulation;
Laser assisted hatching;
Mouse embryo;
Vitrification
- MeSH:
Animals;
Blastocyst;
Blastomeres;
Cell Count;
Embryonic Development*;
Embryonic Structures*;
Female;
Flushing;
Herpes Zoster;
Mice*;
Oviducts;
Pregnancy;
Vitrification*
- From:Clinical and Experimental Reproductive Medicine
2017;44(4):193-200
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. METHODS: First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed. RESULTS: The hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p < 0.05). In the control-8 group (22.1±4.6), the cell number of the inner cell mass was higher than in the LAH groups (p < 0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group (92.8±8.9) than in the others (p < 0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group (19.5±5.1, p < 0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group (73.2±12.1) than in the other groups, but without statistical significance. CONCLUSION: When LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.
