Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation.
10.5653/cerm.2017.44.4.201
- Author:
Sung Woo KIM
1
;
Byung Chul JEE
;
Seul Ki KIM
;
Seok Hyun KIM
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Aneuploidy;
Density gradient centrifugation;
DNA fragmentation;
Sperm;
Swim-up
- MeSH:
Aneuploidy*;
Centrifugation, Density Gradient*;
Chromatin;
DNA Fragmentation*;
DNA*;
Fluorescence;
Humans;
In Situ Hybridization;
Infertility;
Male;
Methods;
Semen;
Sex Chromosomes*;
Spermatozoa*;
Tolonium Chloride;
Y Chromosome
- From:Clinical and Experimental Reproductive Medicine
2017;44(4):201-206
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. METHODS: Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined. RESULTS: The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p=0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p=0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p=0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p=0.316). CONCLUSION: The swim-up method is superior for enriching genetically competent sperm.
