Systemic administration of low dosage of tetanus toxin decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus.
10.5625/lar.2013.29.3.148
- Author:
Bing Chun YAN
1
;
In Hye KIM
;
Joon Ha PARK
;
Ji Hyeon AHN
;
Jeong Hwi CHO
;
Bai Hui CHEN
;
Jae Chul LEE
;
Jung Hoon CHOI
;
Ki Yeon YOO
;
Choong Hyun LEE
;
Jun Hwi CHO
;
Jong Dai KIM
;
Moo Ho WON
Author Information
1. Department of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, China.
- Publication Type:Original Article
- Keywords:
Exotoxin;
neuronal damage;
neurogenesis;
sub-granular zone;
granule cell
- MeSH:
Adult;
Animals;
Bromodeoxyuridine;
Cell Proliferation;
Dentate Gyrus;
Exotoxins;
Fluoresceins;
Gliosis;
Humans;
Immunohistochemistry;
Mice;
Neurogenesis;
Neurons;
Tetanus;
Tetanus Toxin
- From:Laboratory Animal Research
2013;29(3):148-155
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.
