Regenerative Effect of Microsuture Nerve Repair and Fibrin Glue after Rat Sciatic Nerve Section.
- Author:
Bum Tae KIM
1
;
Yong Suck KIM
;
Jae Chil JANG
;
Won Han SHIN
;
Soon Kwan CHOI
;
Bark Jang BYUN
Author Information
1. Department of Neurosurgery, College of Medicine, Soonchunhyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Direct end-to-end suture;
Nerve graft;
Fibrin glue;
Nerve conduction study;
Regenera
- MeSH:
Animals;
Axons;
Fibrin Tissue Adhesive*;
Fibrin*;
Neural Conduction;
Rats*;
Regeneration;
Sciatic Nerve*;
Skull Base;
Sutures;
Transplants
- From:Journal of Korean Neurosurgical Society
1998;27(4):430-437
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To compare the regenerative effect of direct end-to-end suture, nerve graft and sutureless fibrin glue repair in the rat sciatic model, forty rats were divided into 4 groups: normal control group(NC-G, n=6), direct end-to-end suture group(DS-G, n=12), nerve graft group(NG-G, n=10) and sutureless fibrin glue group(FG-G, n=12). One half of each group except NC-G was sacrificed 4 weeks later and the other half was sacrificed 14 weeks later. The repair site was assessed by nerve conduction studies and quantitative morphometry. Regeneration index (RI) was defined as a total number of remyelinated axons in one quarter of 3X5 inch sized microphotograph. The comparison among groups was analyzed statistically by Kruskal-Wallis 1-way ANOVA and Mann-Whitney test. Nerve conduction study done 14 weeks later revealed that latency was 1.7ms+/-0.18(DS-G), 2.4ms+/-0.35(NG-G), 2.4ms+/-0.17(FG-G) and amplitude was 20.1mV+/-2.89(DS-G), 12.0mV+/-2.47(NG-G), 13.7mV+/-2.95(FG-G). RI was 281+/-37.5(DS-G), 227+/-14.4(NG-G) and 217+/-22.2(FG-G). There was a statistical difference in the latency, amplitude and RI between three groups(p<0.05), but there was no statistical difference in the latency, amplitude and RI between FG-G and NG-G(p>0.05). Regenerative effect of fibrin glue is not different from that of nerve graft, therefore fibrin glue repair can facilitate inaccessible nerve repairs in skull base or spinal surgery.
