Transcriptional regulation of Zic3 by heterodimeric AP-1(c-Jun/c-Fos) during Xenopus development.
- Author:
Sung Young LEE
1
;
Hyun Shik LEE
;
Jin Soo MOON
;
Jong Il KIM
;
Jae Bong PARK
;
Jae Yong LEE
;
Mae Ja PARK
;
Jaebong KIM
Author Information
1. Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-do 200-702, Korea. jbkim@hallym.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Activin;
AP-1(c-Jun & c-Fos);
Xenopus development;
Zic3
- MeSH:
Activins/pharmacology/physiology;
Animals;
Base Sequence;
Binding Sites/genetics;
Consensus Sequence/genetics;
Dimerization;
Embryo, Nonmammalian/metabolism;
*Gene Expression Regulation, Developmental;
Homeodomain Proteins/*genetics;
Molecular Sequence Data;
Promoter Regions (Genetics)/genetics;
Proto-Oncogene Proteins c-fos/genetics/physiology;
Proto-Oncogene Proteins c-jun/genetics/physiology;
RNA, Antisense/genetics;
Research Support, Non-U.S. Gov't;
Transcription Factor AP-1/genetics/*physiology;
Transcription Factors/*genetics;
*Transcription, Genetic;
Up-Regulation;
Xenopus Proteins/*genetics;
Xenopus laevis/*embryology/*genetics
- From:Experimental & Molecular Medicine
2004;36(5):468-475
- CountryRepublic of Korea
- Language:English
-
Abstract:
The heterodimeric c-Jun/c-Fos, an activator protein-1 (AP-1) has been implicated in mesoderm induction (Dong et al., 1996; Kim et al., 1998) whereas the homodimer of c-Jun was reported to be involved in neural inhibition during the early development of Xenopus embryos. During the early vertebrate development AP-1 involvement in the neural induction is still not clearly understood. We report here that AP-1 has a role in Zic3 expression, a critical proneural gene and a primary regulator of neural and neural crest development (Nakata et al., 1997; Nakata et al., 1998). AP-1 was able to induce the Zic3 gene in a dose dependent manner but other homo- or hetero-dimeric proteins, such as c-Jun/c-Jun, JunD/FosB or JunD/Fra-1 were not. The inhibition of AP-1 activity using morpholino antisenses of c-jun mRNAs blocked the Zic3 expression induced by activin. In addition, co-injection of c-jun mRNA rescued the down-regulated Zic3 expression. The promoter region of isolated Zic3 genomic DNA was found to possess several consensus-binding site of AP-1. Thus, in the functional assays, AP-1 could increase promoter activity of Zic3 gene. These findings suggest that proneural gene, Zic3 may be regulated by heterodimeric AP-1(c-Jun/c-Fos) and it may have a role in activin signaling for the regulation of neural specific gene, Zic3.
