Immunomodulatory Effects of Dioscoreae Rhizome Against Inflammation through Suppressed Production of Cytokines Via Inhibition of the NF-kappaB Pathway.
- Author:
Seulah KIM
1
;
Seulmee SHIN
;
Bobae HYUN
;
Hyunseok KONG
;
Shinha HAN
;
Aeri LEE
;
Seungjeong LEE
;
Kyungjae KIM
Author Information
- Publication Type:Original Article
- Keywords: Dioscoreae Rhizoma; Anti-inflammatory; Inflammatory cytokines; Immunomodulatory agent
- MeSH: Blotting, Western; Cytokines; Dinoprostone; Dioscorea; Immune System Diseases; Inflammation; Interleukin-10; Interleukin-4; Interleukin-6; Macrophages; Medicine, Traditional; NF-kappa B; Rhizome; RNA, Messenger; Transcription Factors; Tumor Necrosis Factor-alpha; Water
- From:Immune Network 2012;12(5):181-188
- CountryRepublic of Korea
- Language:English
- Abstract: Dioscoreae Rhizome (DR) has been used in traditional medicine to treat numerous diseases and is reported to have anti-diabetes and anti-tumor activities. To identify a bioactive traditional medicine with anti-inflammatory activity of a water extract of DR (EDR), we determined the mRNA and protein levels of proinflammatory cytokines in macrophages through RT-PCR and western blot analysis and performed a FACS analysis for measuring surface molecules. EDR dose-dependently decreased the production of NO and pro-inflammatory cytokines such as IL-1beta, IL-6, TNF-alpha, and PGE2, as well as mRNA levels of iNOS, COX-2, and pro-inflammatory cytokines, as determined by western blot and RT-PCR analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 was also reduced by EDR. Furthermore, activation of the nuclear transcription factor, NF-kappaB, but not that of IL-4 and IL-10, in macrophages was inhibited by EDR. These results show that EDR decreased pro-inflammatory cytokines via inhibition of NF-kappaB-dependent inflammatory protein level, suggesting that EDR could be a useful immunomodulatory agent for treating immunological diseases.
