T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line.
- Author:
Jung Sik KIM
1
;
Dong Chul SHIN
;
Min Yeong WOO
;
Myung Hee KWON
;
Kyongmin KIM
;
Sun PARK
Author Information
- Publication Type:Original Article
- Keywords: T cell immunoglobulin mucin domain-3; TGF-beta1; Transcription; Mast cells; Smad
- MeSH: Cytokines; Humans; Immunoglobulins; Interferons; Interleukin-10; Luciferases; Mast Cells; Mucins; RNA, Messenger; T-Lymphocytes; Transcription Initiation Site; Transforming Growth Factor beta1; Tumor Necrosis Factor-alpha
- From:Immune Network 2012;12(5):207-212
- CountryRepublic of Korea
- Language:English
- Abstract: T cell immunoglobulin mucin domain (TIM)-3 is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Therefore, we examined the transcript level and the promoter activity of TIM-3 in mast cells. The TIM-3 transcript level was assessed by real-time RT-PCR and promoter activity by luciferase reporter assay. TIM-3 mRNA levels were increased in HMC-1, a human mast cell line by TGF-beta1 stimulation but not by stimulation with interferon (IFN)-alpha, IFN-lambda, TNF-alpha, or IL-10. TIM-3 promoter -349~+144 bp region relative to the transcription start site was crucial for the basal and TGF-beta1-induced TIM-3 promoter activities in HMC-1 cells. TIM-3 promoter activity was increased by overexpression of Smad2 and Smad4, downstream molecules of TGF-beta1 signaling. Our results localize TIM-3 promoter activity to the region spanning -349 to +144 bp in resting and TGF-beta1 stimulated mast cells.
