Differentiation of adult stem cell derived from buccal fat pad into osteoblast.
- Author:
Sung Woon PYO
1
;
Jang Woo PARK
;
Il Kyu LEE
;
Chang Hyen KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea. omfskim1@nate.com
- Publication Type:Original Article
- Keywords:
Tissue engineering;
Stem cell;
Buccal fat pad;
Buccal fat derived cell;
Osteoblast
- MeSH:
Adipose Tissue*;
Adult Stem Cells*;
Adult*;
Alkaline Phosphatase;
Bone and Bones;
Bone Transplantation;
Calcium;
Cartilage;
Gene Expression;
Humans;
Mesenchymal Stromal Cells;
Osteoblasts*;
Osteocalcin;
Stem Cells;
Tissue Donors;
Tissue Engineering
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2006;32(6):524-529
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
For the repairing of bone defect, autogenous or allogenic bone grafting remains the standard. However, these methods have numerous disadvantages including limited amount, donor site morbidity and spread of diseases. Tissue engineering technique by culturing stem cells may allow for a smart solution for this problem. Adipose tissue contains mesenchymal stem cells that can be differentiate into bone, cartilage, fat or muscle by exposing them to specific growth conditions. In this study, the authors procured the stem cell from buccal fat pad and differentiate them into osteoblast and are to examine the bone induction capacity. Buccal fat-derived cells (BFDC) were obtained from human buccal fat pad and cultured. BFDC were analyzed for presence of stem cell by immunofluorescent staining against CD-34, CD-105 and STRO-1. After BFDC were differentiated in osteogenic medium for three passages, their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase (ALP) staining, Alizarin red staining and RT-PCR for osteocalcin (OC) gene expression. Immunofluorescent and biochemical assays demonstrated that BFDC might be a distinguished stem cells and mineralization was accompanied by increased activity or expression of ALP and OC. And calcium phosphate deposition was also detected in their extracelluar matrix. The current study supports the presence of stem cells within the buccal fat pad and the potential implications for human bone tissue engineering for maxillofacial reconstruction.
