Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.
10.3346/jkms.2016.31.2.315
- Author:
Wan Beom PARK
1
;
Nak Jung KWON
;
Pyoeng Gyun CHOE
;
Su Jin CHOI
;
Hong Sang OH
;
Sang Min LEE
;
Hyonyong CHONG
;
Jong Il KIM
;
Kyoung Ho SONG
;
Ji Hwan BANG
;
Eu Suk KIM
;
Hong Bin KIM
;
Sang Won PARK
;
Nam Joong KIM
;
Myoung Don OH
Author Information
1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. mdohmd@snu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Middle East Respiratory Syndrome;
Middle East Respiratory Syndrome Coronavirus;
Microscopy, Electron;
Phylogeny;
Korea
- MeSH:
Animals;
Cercopithecus aethiops;
Coronavirus Infections/*diagnosis/epidemiology/*virology;
Disease Outbreaks;
Humans;
Microscopy, Electron;
Middle East Respiratory Syndrome Coronavirus/classification/genetics/*isolation & purification/ultrastructure;
Phylogeny;
Polymerase Chain Reaction;
RNA, Viral/analysis/chemistry/metabolism;
Republic of Korea/epidemiology;
Sequence Analysis, RNA;
Vero Cells
- From:Journal of Korean Medical Science
2016;31(2):315-320
- CountryRepublic of Korea
- Language:English
-
Abstract:
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
