Allele Distribution and Frequency of Human Surfactant Protein-A1 in Korean Neonates.
- Author:
Kyung Shin LEE
1
;
Young Hee KIM
;
Jung Su SUK
;
Jung Ho KO
;
Ook Joon YOO
;
In Kyu LEE
;
Myung Ho OH
;
Chong Woo BAE
Author Information
1. Department of Pediatrics, College of Medicine, Soonchunhyang University, Chonan, Korea. omh@schch.co.kr
- Publication Type:Original Article
- Keywords:
Surfactant;
SP-A1;
SP-A1 alleles;
RDS;
Steroid
- MeSH:
Alleles*;
DNA;
Gene Frequency;
Genotype;
Humans*;
Infant, Newborn*;
Korea;
Polymerase Chain Reaction;
Prevalence
- From:Journal of the Korean Pediatric Society
2002;45(12):1497-1502
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We evaluated allele frequencies and distribution of surfactant protein A1(SP-A1) in Korean neonates in order to estimate prevalence of RDS to find out new SP-A alleles, and to establish new steroid therapy. METHODS: Genomic DNA was extracted from 100 neonates and served as a template in PCR for genotype analysis. SP-A gene-specific amplications and gene-specific allele determinations were performed using PCR-RFLP methods. RESULTS: The distribution for the alleles of the SP-A1 gene in the study population were 6A, 6A(2), 6A(3), 6A(4), 6A(8), 6A(9), 6A(10), 6A(11), 6A(12), 6A(13), 6A(14), 6A(15), 6A(16), 6A(17), 6A(18), 6A(20). The specific frequencies for the alleles of the SP-A1 gene in the study population were: 6A(2)=21%, 6A(3)=45%, 6A(4)=11%, 6A(8)=9%, 6A(14)=8%. CONCLUSIONS: The frequency of 6A3 was higher than the other SP-A1 alleles in Korean neonates. This finding suggests that the prevalence of RDS in Korea may be low compared with other countries. However, this finding also suggests that Korean neonates have a high risk of infection.
