A Study of the Pathway of Nitric Oxide Production by Nitroglycerin in Trabecular Meshwork Cells.
10.3341/jkos.2013.54.9.1429
- Author:
Suk Hyun YOON
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
eNOS;
Nitroglycerin;
Nitric oxide;
Trabecular meshwork cells
- MeSH:
Humans;
Nitric Oxide;
Nitroglycerin;
Phosphatidylinositols;
Phosphotransferases;
RNA, Messenger;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2013;54(9):1429-1434
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of nitroglycerin on the production of nitric oxide and its related pathway in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 10 nM nitroglycerin using 1% serum-containing media for 30 minutes. The production of nitric oxide was assessed with the Griess assay and expressions of eNOS mRNA was assessed with RT-PCR. Additionally, the cells were exposed to wortmanin and Akt1/2 kinase inhibitor to investigate the mechanism related to the production of nitric oxide. RESULTS: Nitroglycerin increased the production of nitric oxide (p < 0.05) accompanied with increased expression of eNOS mRNA. The increased production of nitric oxide and eNOS mRNA was inhibited by wortmanin and Akt1/2 kinase inhibitor. CONCLUSIONS: Low-dose nitroglycerin increased the production of nitric oxide accompanied by increased eNOS activity. Nitroglycerin drives eNOS activation via the phosphatidylinositol 3-kinase/protein kinase B pathway.
