Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms.
10.3347/kjp.2015.53.4.483
- Author:
Danielle KIAN
1
;
Cesar Armando Contreras LANCHEROS
;
Igor Alexandre Campos DAMIANI
;
Tamiris Zanforlin Olmos FERNANDES
;
Phileno PINGE-FILHO
;
Marcia Regina Machado DOS SANTOS
;
Jose Franco DA SILVEIRA
;
Celso Vataru NAKAMURA
;
Joao Santana DA SILVA
;
Sueli Fumie YAMADA-OGATTA
;
Lucy Megumi YAMAUCHI
Author Information
1. Departamento de Microbiologia, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Londrina, PR, Brazil. lionilmy@uel.br
- Publication Type:Brief Communication
- Keywords:
Trypanosoma cruzi;
Tc8.2 expression;
dual localization
- MeSH:
Amino Acid Sequence;
Chagas Disease/*parasitology;
Gene Expression Regulation, Developmental;
Humans;
Life Cycle Stages;
Molecular Sequence Data;
Protozoan Proteins/*genetics/metabolism;
Sequence Alignment;
Trypanosoma cruzi/*genetics/*growth & development/isolation & purification/metabolism
- From:The Korean Journal of Parasitology
2015;53(4):483-488
- CountryRepublic of Korea
- Language:English
-
Abstract:
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
