The Role of the Helicobacter pylori CagA Gene in Gastric Cancer Tissue of Koreans.
- Author:
Han Chu LEE
1
;
Sun Young YI
;
Sung Ae JUNG
;
Il Hwan MOON
;
Hye Kyung JUNG
;
Doe Young KIM
Author Information
1. Department of Internal Medicine, Ewha Womans University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Helicobacter pylori;
CagA gene
- MeSH:
Biopsy;
Helicobacter pylori*;
Helicobacter*;
Humans;
Polymerase Chain Reaction;
Stomach Neoplasms*
- From:Korean Journal of Gastrointestinal Endoscopy
1999;19(4):550-556
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND AIMS: To further understand the relationship between the cagA gene and gastric cancer, the positive rates of the cagA gene in cancer and non-cancer tissues were investigated separately in patients with gastric cancer. METHODS: The cagA gene was detected by PCR and the ureC gene was analyzed as a positive control for the presence of Helicobacter pylori. Each of two endoscopic biopsies were obtained from cancer and non-cancer tissues of 41 patients with gastric cancer. RESULTS: 1) The positive rate of the cagA gene in cancer tissues was 29.3% (12/41), which was significantly lower than that in non-cancer tissues (63.4%). 2) Twelve (29.3%) out of 41 were positive for the cagA gene in both cancer and non-cancer tissues, 14 were positive in only non-cancer tissues, none were positive in only cancer tissues, and 15 (36.6%) were negative in both sites. 3) The ureC gene was negative in cancer tissue in 12 (85.7%) among 14 cases who were cagA gene negative in the cancer tissue but positive in the non-cancer tissue. 4) There was no difference in the positive rate of the cagA gene according to age, stage, site, and pathologic cell type. CONCLUSIONS: These findings indicate that the positive rate of the cagA gene in cancer tissue was lower than that in non-cancer tissues and this might be related to a low infection rate of H. pylori in cancer tissue rather than the presence of cagA negative H. pylori in cancer tissues.
