Schedule-Dependent Effect of Epigallocatechin-3-Gallate (EGCG) with Paclitaxel on H460 Cells.
10.4046/trd.2014.76.3.114
- Author:
Sunghoon PARK
1
;
Joo Hee KIM
;
Yong Il HWANG
;
Ki Suck JUNG
;
Young Sook JANG
;
Seung Hun JANG
Author Information
1. Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Korea. chestor@hallym.or.kr
- Publication Type:Original Article
- Keywords:
Epigallocatechin Gallate;
Lung Neoplasms;
Paclitaxel;
Cell Cycle
- MeSH:
Apoptosis;
Blotting, Western;
Carcinoma, Non-Small-Cell Lung;
Caspase 3;
Cell Cycle;
Cell Cycle Checkpoints;
Humans;
Lung Neoplasms;
Models, Animal;
Paclitaxel*;
Poly(ADP-ribose) Polymerases;
Tea
- From:Tuberculosis and Respiratory Diseases
2014;76(3):114-119
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Epigallocatechin-3-gallate (EGCG), a major biologically active component of green tea, has anti-cancer activity in human and animal models. We investigated the schedule-dependent effect of EGCG and paclitaxel on growth of NCI-H460 non-small cell lung cancer cells. METHODS: To investigate the combined effect of EGCG (E) and paclitaxel (P), combination indices (CIs) were calculated, and cell cycle analysis was performed. For the effect on cell apoptosis, western blot analysis was also performed. RESULTS: CI analysis demonstrated that both concurrent and sequential E --> P treatments had antagonistic effects (CIs >1.0), but sequential P --> E had synergistic effects (CIs <1.0), on the growth inhibition of NCI-H460 cells. In the cell cycle analysis, although paclitaxel induced G2/M cell cycle arrest and increased the sub-G1 fraction, concurrent EGCG and paclitaxel treatments did not have any additive or synergistic effects compared with the paclitaxel treatment alone. However, western blot analysis demonstrated that sequential P --> E treatment decreased the expression of Bcl-2 and procaspase-3 and increased poly(ADP-ribose) polymerase (PARP) cleavage; while minimal effects were seen with concurrent or sequential E --> P treatments. CONCLUSION: Concurrent or sequential E --> P treatment had opposite effects to P --> E treatment, where P --> E treatment showed a synergistic effect on growth inhibition of NCI-H460 cells by inducing apoptosis. Thus, the efficacy of EGCG and paclitaxel combination treatment seems to be schedule-dependent.
