Mast Cells in Renal Allografts.
- Author:
Jae Choon LEE
1
;
Samuel LEE
;
Sung Gil PARK
;
Seung Il KIM
;
Young Cheol LEE
;
Joo Seop KIM
;
Sun Hyung JOO
;
Kook Hwan OH
;
Dong Wan CHAE
;
Eun Sook NAM
;
Chul Jae PARK
Author Information
1. Department of Surgery, Hallym University College of Medicine, Seoul, Korea. slee@hallym.or.kr
- Publication Type:Original Article
- Keywords:
Renal transplantation;
Chronic rejection;
Mast cells
- MeSH:
Allografts*;
Biopsy;
Blood Pressure;
Cadaver;
Cholesterol;
Cyclosporine;
Female;
Fibrosis;
Humans;
Hypercholesterolemia;
Immunohistochemistry;
Inflammation;
Kidney Transplantation;
Mast Cells*;
Necrosis;
Tissue Donors;
Tryptases
- From:Journal of the Korean Surgical Society
2002;63(3):201-205
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Chronic rejection is the enemy in the battle for long term survival after renal allografts. Interstitial fibrosis is known to be the important finding in renal allografts with chronic rejection. Mast cells secrete a large number of fibrogenic factors and have been involved in chronic inflammation and tissue fibrosis. In this study the authors evaluated the relationship between mast cells and fibrosis in renal allografts with chronic rejection. METHODS: The authors evaluated 42 biopsied specimens of renal allografts. Immunohistochemistry using anti-mast cell tryptase (Dako, 1 : 200) and an LSAB kit (Dako) was applied to detect mast cells. The mean number of mast cells (MNM) per 10 high power fields was counted. RESULTS: MNM of implantation biopsies was 0.640+/-0.537, of acute rejection -1.969+/-1.216, of chronic rejection -6.0+/-3.133 (P<0.01), of acute tubular necrosis -1.360+/-0.899, and of acute cyclosporine nephrotoxicity -1.000+/-0.600. MNM according to donor source was 3.267+/-3.479 vs. 2.376+/-1.900 (living donors vs. cadaveric donors). MNM was significantly correlated with donor sex (male : female ratio of = 2.319+/-1.739 : 4.014+/-4.286, P<0.01), and cholesterol (hypercholesterolemia vs. non-hypercholesterolemia, 4.125+/-5.497 vs. 2.60+/-1.916, P<0.01). However, MNM according to blood pressure was not statistically significant (hypertension : non-hypertension ratio of=3.189+/-3.05 : 1.200+/-1.226, P>0.05). CONCLUSION: Our data show that the number of mast cells in renal allograft was significantly associated with chronic rejection, donor sex and hypercholesterolemia.
