Immunohistochemical Study of Central Neurocytoma, Subependymoma and Subependymal Giant cell Astrocytoma Located around the Foramen of Monro.
- Author:
So Hyang IM
1
;
Jung Eun KIM
;
Sun Ha PAEK
;
Yoon Ra CHOI
;
Gee Young CHOE
;
Je G CHI
;
Hae Young SUH
;
Dong Gyu KIM
;
Hee Won JUNG
Author Information
1. Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Central neurocytoma;
Subependymoma;
Subependymal giant cell astrocytoma;
Immunohistochemistry;
Central neurocytoma;
Tumor origin
- MeSH:
Antibodies, Monoclonal;
Astrocytoma*;
Cerebral Ventricles*;
Chromogranin A;
Cytoplasm;
Giant Cells;
Glioma, Subependymal*;
Immunohistochemistry;
Nestin;
Neural Cell Adhesion Molecules;
Neurocytoma*;
Neurons;
Phosphopyruvate Hydratase;
RNA-Directed DNA Polymerase;
Synaptophysin
- From:Journal of Korean Neurosurgical Society
2002;31(6):517-523
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECT: To gather information concerning ontogeny, the authors present the results of immunohistochemical stainings of neuronal and glial markers and the reverse transcriptase-prolongation chain reaction (RT-PCR) of nestin for three intraventricular tumors located around the foramen of Monro. METHODS: Seven cases of central neurocytomas(CN), three subependymomas(SE) and eight subependymal giant cell astrocytomas(SEGA), were included in this study. Antihuman monoclonal antibodies of synaptophysin(SNP)(DAKO, 1:20), chromogranin A(ChrA)(DAKO, 1:100), neuron specific enolase (NSE)(DAKO, 1:500) and nerve cell adhesion molecule(NCAM)(Zymed, 1:500) were utilized for neuronal markers and glial fibrillary acidic protein(GFAP)(DAKO, 1:300) functioned as a glial marker in immunohistochemical(IHC) stainings. Reverse transcriptase polymerase chain reaction(RT-PCR) for nestin was performed in all cases. RESULTS: For chromogranin A, positive reaction was found in three of the seven CN cases but none of the SE and SEGA cases. For IHC staining of synaptophysin, positive reaction was revealed in all CN cases but in none of the SE and SEGA cases. For NCAM, positive reaction was demonstrated in five of the eight SEGA cases and in all SE and CN cases. For NSE, positive reaction was exhibited in seven of the eight SEGA cases and in all SE and CN cases. Positive reactions for NSE and NCAM in the SEGA cases were manifested mainly in the cytoplasms of giant cells and their background. For IHC staining of GFAP, positive reaction was demonstrated in one of the seven CN cases, in three of the eight SEGA cases, and in all SE cases. RT-PCR product of nestin was expressed in two of the seven CN cases, in two of the three SE cases, and in one SEGA case. CONCLUSION: Many cells of CN, SE and SEGA, had expressed positive reactions for both neuronal and glial markers in IHC study and nestin in RT-PCR. It is suggested that origin cells of these tumors might express both neuronal and glial differentiation.
